Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)
HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.
Alkali Scientific MOPSO Sodium Salt, 2 Kg
MOPSO Sodium Salt is a buffering reagent used to stabilize pH in laboratory applications. The 2 kg weight offers ample supply for large-scale experiments, ensuring consistency and reproducibility in analytical procedures. This sodium salt of MOPSO (Morpholinopropanesulfonic acid) provides excellent buffering capacity, especially in the pH range of 7.0–8.5, which is critical for maintaining the integrity of sensitive biological samples and chemical reactions. MOPSO is frequently used in the preparation of buffers for chromatography, electrophoresis, and enzyme assays. Its ability to maintain pH stability in various solutions makes it an essential reagent for biochemical and pharmaceutical applications.
Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at approximately pH 6.15
- Poor affinity with metal ions
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005335 HEPES EMPROVE EVOLVE 100G
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659782.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical buffer available in USP-grade, designed for a wide range of scientific and research applications. This 5kg container is ideal for laboratories that require large volumes of buffer solution for high-throughput processes, including protein studies, enzymatic assays, and cell culture applications. PIPES is known for its ability to maintain a stable pH under various conditions where other buffers may not be suitable. It is especially effective in high-salt and high-temperature environments. The 5kg pack ensures that large-scale laboratory projects can proceed without the need for frequent reordering, making it both a cost-effective and convenient solution.
Sigma Aldrich Fine Chemicals Biosciences POPSO hydrate >=99% (titration) | 68189-43-5 (anhydrous) | 100G
POPSO hydrate >=99% (titration) | Purity: >=99% (titration) | 68189-43-5 (anhydrous) | 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul50G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Medchemexpress LLC MOPS sodium salt | 71119-22-7 | 99.8% | 500 G
MOPS sodium salt is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It significantly improves the thermal stability of BSA. MOPS sodium salt is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.
- Purity: 99.8%
- Appearance: Solid
- Color: White to off-white
- Storage: 4°C, sealed, away from moisture. In solvent: -80°C for 6 months; -20°C for 1 month (sealed, away from moisture).
- Solubility in H2O: 100 mg/mL (needs ultrasonic)
- Solubility in DMSO: 50 mg/mL (needs ultrasonic; use newly opened DMSO)
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 49 Grams of Powder, Makes 10 Liters of Solution
Murashige & Skoog Medium with MES Buffer and Vitamins (10 liters, 49 grams of powder) is designed for plant tissue culture applications that require large volumes of prepared solution. This medium is enriched with essential nutrients, growth regulators, and MES buffer, which helps to stabilize the pH during plant tissue growth. The formula ensures optimal growth conditions for plant cells, tissues, and organs in vitro, making it ideal for shoot multiplication, regeneration, and propagation experiments. It is commonly used in commercial plant propagation, agricultural research, and plant breeding. The large 10-liter yield makes this medium suitable for labs and research facilities with higher throughput needs.
Research Products International Corp MES, Anhydrous, 1 Kilograms
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp PIPES Sesquisdm Salt 250G
PIPES Sesquisodium Salt, also known as PIPES Na3, is a chemical compound that has significant applications in the life science marketplace. PIPES stands for piperazine-N,N'-bis(2-ethanesulfonic acid), and the sesquisodium salt refers to the trisodium salt form of PIPES.
It is primarily utilized as a buffering agent. Buffers are substances that help maintain a stable pH in biological and biochemical processes, ensuring optimal conditions for various enzymatic reactions and cellular activities.
PIPES Sesquisodium Salt is particularly valuable in biological research, molecular biology, and cell culture applications. It offers excellent buffering capacity in the physiological pH range (6.1 - 7.5), making it suitable for a wide range of experimental conditions.
eMolecules 58884-35-8 | 4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenol | Broadpharm | 58884-35-8 | MFCD24716961 | 188.190 | C9H8N4O | 98.000 | Cc1nnc(nn1)-c1ccc(O)cc1 | 1g | 349512533
Research Products International Corp PIPES FREE ACID ULTRAPURE, 500GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5