Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92060-25.0.JPG-250.jpg)
Research Products International Corp MOPSO [3-(N-Morpholino)-2-hydroxypropanesulfonic acid], 25 Grams
- Substance Name: 3-(N-Morpholino)2-hydroxypropanesulfonic acid
- CAS Number: 68399-77-9
- Molecular Formula: C7H15NO5S
- Molecular Weight: 225.3
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.5, 100 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.5
Chemscene ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 10G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 10G
![Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-C40020-500.0.JPG-250.jpg)
Research Products International Corp CHES [2-(N-Cyclohexylamino)ethanesulfonic acid], 500 Grams
CHES, also known as 2-(N-Cyclohexylamino) ethanesulfonic acid, is a chemical compound that plays a significant role in life science research, particularly in molecular biology and protein research.
CHES is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 8.6 to 10.0, making it suitable for applications requiring higher alkaline pH conditions.
CHES is also commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability in alkaline conditions make it valuable in these applications.
One of the significant advantages of CHES is its low ionic strength, which minimizes interference with biological processes and enzymatic activities.
Dojindo Molecular Technologies Inc TAPSO (25G), Good's buffer, CAS: 68399-81-5, pka=7.7, Active pH 7.0-8.2, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255317.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 250 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Chem-Impex International, Inc. Bicine | 150-25-4 | MFCD00004295 | 100G
Bicine, 150-25-4, MFCD00004295, 100G
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Boston Bioproducts Inc MOPS Buffer (1 M, pH 8.0) - 500ml
MOPS Buffer (1 M pH 8 0) - 500ml (For Research Use and Further Manufacturing Only)
Chem-Impex International, Inc. N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid | MFCD00007532 | 250G
N-tris(Hydroxymethyl)methyl-2-aminoethane-sulfonic acid, MFCD00007532, 250G
Sigma Aldrich Fine Chemicals Biosciences MOPSO >=99% (titration) | 68399-77-9 | MFCD00009629 | 100G
MOPSO >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 225.26 | 68399-77-9 | MFCD00009629 | 100G
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 1KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 1KG
